Effect of Growth Factors on Cell Proliferation, Matrix Deposition, and Morphology of Human Nasal Septal Chondrocytes Cultured in Monolayer

Autor: Richmon, Jeremy D., Sage, August B., Shelton, Elliot, Schumacher, Barbara L., Sah, Robert L., Watson, Deborah
Zdroj: The Laryngoscope; September 2005, Vol. 115 Issue: 9 p1553-1560, 8p
Abstrakt: Objectives:Tissue engineering of septal cartilage provides ex vivo growth of cartilage from a patient's own septal chondrocytes for use in craniofacial reconstruction. To become clinically applicable, it is necessary to rapidly expand a limited population of donor chondrocytes and then stimulate the production of extracellular matrix on a biocompatible scaffold. The objective of this study was to determine favorable serum‐free culture conditions for proliferation of human septal chondrocytes using various concentrations and combinations of four growth factors.
Databáze: Supplemental Index