| Autor: |
Vives, Joaquim, Juanola, Sandra, Cairó, Jordi J., Prats, Eva, Cornudella, Lluís, Gòdia, Francesc |
| Zdroj: |
Biotechnology Progress; 2003, Vol. 19 Issue: 1 p84-89, 6p |
| Abstrakt: |
Targets for metabolic engineering have been identified in a hybridoma cell line to make it more robust in culture toward potential limitations inducing apoptosis. The cells were genetically modified with plasmids harboring endogenous bcl‐2gene and also with viral Bcl‐2 homologues, particularly ksbcl‐2and bhrf‐1genes. When cells were exposed to apoptosis‐inducing conditions (i.e., glutamine‐free medium), the control cells exhibited a decrease in viable cell number within the first 12 h, whereas, for the bcl‐2and ksbcl‐2transfected cell cultures, the viable cell number did not exhibit any clear decrease until after 60 h. Furthermore, hybridoma cells expressing the viral homologue bhrf‐1were even more resistant to cell death, showing a decrease in viability of only 50% at 72 h of culture in glutamine‐deprived medium, substantially lower than the 90% viability decrease observed for the control culture. In addition, and most relevant for further bioprocess applications, the cells genetically modified could be brought back to growth conditions even after being exposed to glutamine‐deprived conditions during a significant time window, up to 72 h. |
| Databáze: |
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