| Autor: |
Roberts, David M., Pronobis, Mira I., Poulton, John S., Waldmann, Jon D., Stephenson, Elise M., Hanna, Shahnaz, Peifer, Mark |
| Zdroj: |
Molecular Biology of the Cell; June 2011, Vol. 22 Issue: 11 p1845-1863, 19p |
| Abstrakt: |
Negatively regulating signaling by targeting key effectors for ubiquitination/destruction is essential for development and oncogenesis. The tumor suppressor adenomatous polyposis coli (APC), an essential negative regulator of Wnt signaling, provides a paradigm. APC mutations occur in most colon cancers. Acting in the "destruction complex" with Axin, glycogen synthase kinase 3, and casein kinase, APC targets sscatenin (sscat) for phosphorylation and recognition by an E3 ubiquitin-ligase. Despite 20 years of work, the internal workings of the destruction complex and APC's role remain largely mysterious. We use both Drosophila and colon cancer cells to test hypotheses for APC's mechanism of action. Our data are inconsistent with current models suggesting that high-affinity sscat-binding sites on APC play key roles. Instead, they suggest that multiple sscat-binding sites act additively to fine-tune signaling via cytoplasmic retention. We identify essential roles for two putative binding sites for new partners—20-amino-acid repeat 2 and conserved sequence B—in destruction complex action. Finally, we demonstrate that APC interacts with Axin by two different modes and provide evidence that conserved sequence B helps ensure release of APC from Axin, with disassembly critical in regulating sscat levels. Using these data, we suggest a new model for destruction complex action in development, which also provides new insights into functions of truncated APC proteins in cancer. |
| Databáze: |
Supplemental Index |
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