| Abstrakt: |
The uptake of live and heat‐killed Malassezia furfurHIC 3321, HIC 3343 and Candida albicansATCC 10231 by human monocytic cell line, THP‐1, was examined. THP‐1 was differentiated by PMA for 7 days before use. The uptake of these yeasts by THP‐1 was increased in a concentration‐dependent manner of yeasts, and the uptake reached plateau level at the E/T (yeast/THP‐1) ratio 5. In addition, a higher percentage of heat‐killed cells than live cells was taken in THP‐1. Yeast mannan and β‐1,3‐glucan, random coiled conformer, inhibited the uptake of live and heat‐killed M. furfurby THP‐1, though dextran T‐250, that is α‐glucan, and schizophyllan (SPG), triple helix conformer of β‐glucan, did not. Interestingly, mannan inhibited the uptake of both types, live and heat‐killed, of C. albicans, however, laminaran inhibited the uptake of heat‐killed C. albicansalone. Opsonization of these yeasts with normal human serum enhanced the uptake of yeasts, although opsonization with heat‐inactivated serum, the treatment at 56°C for 30 min, did not enhance. These results suggested that live and heat‐killed M. furfurwas recognized by THP‐1 through mannose receptor, β‐glucan receptor and complement receptor type 3 via the activation of alternative pathway of complement. |
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