| Autor: |
Saruta, Katsutoshi, Matsunaga, Teiichi, Kono, Midori, Hoshina, Sadayori, Kanemoto, Sonomi, Sakai, Osamu, Machida, Katsuhiko |
| Zdroj: |
FEMS Immunology and Medical Microbiology; October 1997, Vol. 19 Issue: 2 p151-157, 7p |
| Abstrakt: |
Haemophilus influenzaeand Streptococcus pneumoniaeare often the cause of serious diseases such as meningitis. We designed a nested PCR assay to identify these pathogens from cerebrospinal fluid samples. The first‐step PCR was able to detect eubacterial rRNA genes with a unified set of universal primers. In the second‐step PCR, the identification primers, HI I and II and SP I and II, could detect H. influenzaeand S. pneumoniaerespectively through amplification of the rRNA spacer between the 16S and 23S rRNA genes. We suggest that the two‐step PCR assay can be used as a novel method for the immediate and retrospective diagnosis of bacterial meningitis caused by H. influenzaeand S. pneumoniae. |
| Databáze: |
Supplemental Index |
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