Binding of 5-hydroxytryptamine analogs by isolated Spisula sperm membrane

Autor: BANDIVDEKAR, A.H., SEGAL, S.J., KOIDE, S.S.
Zdroj: Invertebrate Reproduction & Development; February 1992, Vol. 21 Issue: 1 p43-46, 4p
Abstrakt: Serotonin receptors were identified in Spisula sperm membranes by radioligand binding assay. Sperm membranes were prepared by nitrogen cavitation and by density gradient ultracentrifugation. Specific binding of [3H]5-HT to the receptors of isolated sperm membranes was determined. The dissociation constant was 2.7 nM and the maximum binding capacity was 11.25 pmoles/mg of protein. The order of decreasing potency of 5-HT agonists in blocking [3H]5-HT binding to the sperm membrane was as follows: 2-methyl 5-HT> 8-OH-DPAT> 5-HT> 5-CT> α-methyl-5-HT; and that of 5-HT antagonists was: ICS-205-930> BMY-7379> mianserin> methysergide. These findings suggest that the 5-HT receptors in Spisula sperm membranes contain sites that interact with 5-HT3 and 5-HT1 analogs.
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