| Autor: |
SAUMAN, IVO, WATSON, CORNELIUS, BERRY, SPENCER |
| Zdroj: |
Invertebrate Reproduction & Development; 1992, Vol. 22 Issue: 1-3 p57-65, 9p |
| Abstrakt: |
The laser-scanning confocal microscope employed in conjunction with various specific agents and antibodies conjugated to fluorescent dyes reveals details of the actin scaffolding of developing oocytes and the nuclei of attendant cells. The employment of DNase I followed by anti-DNase I antibody has been particularly useful in revealing otherwise cryptic actin-containing structures. The cortical cytoskeleton of developing moth eggs was found to bind both poly (A)+RNA and RNA Pol II. Exposure to cytochalasin D disrupted the actin of the cortex, and at the same time caused redistribution of the proteins and RNA associated with the cytoskeleton. Cytochalasin also had dramatic effects on the structure of nuclei of nurse and follicle cells. Taken in context of the actin network in nuclei uncovered by DNase-anti-DNase treatment, these results suggest that actin plays a major structural and perhaps functional role in insect nuclei. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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