| Autor: |
Peter, Karin, Korfmann, Gisela, Wiedemann, Bernd |
| Zdroj: |
Clinical Infectious Diseases; July 1988, Vol. 10 Issue: 4 p800-800, 1p |
| Abstrakt: |
In an investigation of the influence of the ampD gene on β-lactamase production and induction in Enterobacter cloacae, the ampR-ampC gene region cloned into a plasmid and the ampD gene cloned into another vector were transferred to a strain of Escherichia coli. The genetically manipulated E. coli strains served as a model for study of the inducibility of β-lactamases in E. cloacae. In addition, β-lactamase induction in E. cloacae bearing the previously mentioned plasmids was studied. After induction of the β-lactamase with cefoxitin, the specific hydrolytic activity, the viable cell count, and the degradation of cefoxitin were determined. β-Lactamase expression decreased with an increasing amount of the ampD gene product. The cefoxitin concentration decreased in proportion to the amount of enzyme, but the induction of β-lactamase seemed not to be an important factor influencing the viable cell count of E. cloacae as long as cefoxitin concentrations exceeded the MIC. Despite different β-lactamase concentrations, the decrease in the viable cell count was nearly identical in all experiments. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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