| Autor: |
Fulton, S. A., Cross, J. V., Toossi, Z. T., Boom, W. H. |
| Zdroj: |
The Journal of Infectious Diseases; October 1998, Vol. 178 Issue: 4 p1105-1105, 1p |
| Abstrakt: |
Regulation of interleukin (IL)-12 production by coexpression of tumor necrosis factor (TNF)-α, IL-10, and transforming growth factor (TGF)-β in human monocytes infected with Mycobacterium tuberculosis H37Ra was analyzed. Also, since IL-12 induces interferon (IFN)-γ, the effect of IFN-γ on IL-12 expression was examined. IL-12 mRNA was measured by reverse transcriptase-polymerase chain reaction and IL-12 protein by ELISA. IL-12 p35 mRNA was constitutive and inducible. IL-12 p70 protein paralleled IL-12 p40 protein expression. TNF-α protein expression occurred earlier than IL-12 p40 protein but was not required for IL-12 induction. Addition or neutralization of TGF-β did not significantly alter IL-12 induction. In contrast, recombinant IL-10 reduced IL-12 and neutralization of IL-10 minimally enhanced IL-12. A pronounced increase in IL-12 followed IFN-γ pretreatment, which selectively up-regulated IL-12 p35 mRNA. Further understanding of operative cytokine networks during M. tuberculosis infection may improve strategies for vaccine development and immunotherapy. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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