| Autor: |
Bunkin, Aleksei F, Pershin, S M |
| Zdroj: |
Quantum Electronics; December 2010, Vol. 40 Issue: 12 p1098-1102, 5p |
| Abstrakt: |
Four-wave mixing (FWM) spectroscopy is used to detect the rotational resonances of H2O and H2O2 molecules in DNA and denatured DNA aqueous solutions in the range +-10 cm-1 with a spectral resolution of 3 GHz. It is found that the resonance contribution of the rotational transitions of these molecules increases significantly in solutions rather than in distilled water. This fact is interpreted as a manifestation of specific properties of a hydration layer at DNA--water and denatured DNA--water interfaces. Analysis of the FWM spectra shows that the concentration of H2O2 molecules in the hydration layer of the DNA solution increases by a factor of 3 after denaturation. The FWM spectra of aqueous solutions of a-chymotrypsin protein are obtained in the range +-7cm-1 at the protein concentrations between 0 and 20 mg cm-3. It is found that the hypersound velocity in the protein aqueous solution, measured by the shift of Brillouin components in the scattering spectrum, obeys a cubic dependence on the protein concentration and reaches a value of about 3000 m s-1 at 20 mg cm-3. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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