| Autor: |
ARNESEN, Steen, ERIKSEN, Susanne H., OLSEN, Jørgen |
| Zdroj: |
Mycological Research; March 2002, Vol. 106 Issue: 3 p345-348, 4p |
| Abstrakt: |
The thermophilic fungus Thermomyces lanuginosus was cultivated in shake-flasks for up to 120 h with low-molecular-weight dextran as carbon source. Maximal biomass was attained after 48 h of growth whereas extracellular α-amylase activity was highest in the stationary phase with maximum activity after 96 h of cultivation. A similar pattern was found for total extracellular protein. Pulse-labeling of proteins showed that α-amylase and other unidentified proteins were de novo synthesised in the stationary phase. Using specific primers with sequences from α-amylase from T. lanuginosus, RT-PCR analysis showed that α-amylase transcription did not start until late in the growth phase and reached a maximum more than 24 h after maximal biomass was obtained. |
| Databáze: |
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