| Autor: |
Wiemann, M., Erlinghagen, C., Bruch, J., Rehn, B. |
| Zdroj: |
Materialwissenschaft und Werkstofftechnik; December 2010, Vol. 41 Issue: 12 p1086-1092, 7p |
| Abstrakt: |
Particles adsorb biomolecules such as proteins and lipids from biological fluids. Accordingly, respirable particles (aerodynamic diameter <3 μm) inside lung alveolae bind molecules from lung surfactant (LS) which is composed of lipids (90%) and surfactant proteins (10%). In this paper we show that particles impair lung function by adsorbing LS. To this aim, we suspended particles characterized by different surface sizes such as quartz DQ12 and amorphous silica HDKN20 in 0.9% NaCl and tested the effect of a particle containing fluid bolus on the compliance of the isolated rat lung in the presence and absence of surfactant. A method was deviced in which the left lung of the rat was ventilatedex vivoby periodic negative pressure pulses. As an indirect measure of lung compliance the maximal expiratory volume flow of the isolated lung (MEF‐IL) was measured. Experiments were carried out for 90 min during which MEF‐IL showed a slight run down by about 20%. Instillation of 0.9% NaCl further reduced MEF‐IL; this effect was attenuated if lung lavage fluid was used and was completely reversed by commercially available surfactant. Instillation of quartz DQ12 (5 mg) and HDK N20 (2 mg) suspended in 0.9% NaCl lowered MEF‐IL within 10 min by 50.3% and 58.2%, respectively. In both cases pre‐coating with or subsequent addition of surfactant abrogated the inhibitory effect of particles on MEF‐IL. Experiments show that suspended particles affect lung compliance by adsorbing surfactant and that this effect can be quantified by an ex vivo lung model. |
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