| Autor: |
Dalmazio, S., Cammarata, M., Carrillo, D., Calvo, S., Romancino, D., Cognetti, G., Di Carlo, M. |
| Zdroj: |
Russian Journal of Genetics; June 2002, Vol. 38 Issue: 6 p684-690, 7p |
| Abstrakt: |
The seagrass Posidonia oceanicaplays a multifunctional role in the coastal area as an important and productive component of ecosystems in the Mediterranean Sea. We detected by RAPD analysis with two arbitrary primers genetic differences in P. oceanicacollected from several sites in the Southern Mediterranean. By AMOVA analysis we observed a level of about 20% genetic difference among individuals within a population and 80% among populations. A common band of 200 bp was found in all the amplified samples. Cloning and sequencing analysis of this band revealed the presence of a simple tandem repeat sequence (minisatellite) that we called PoTR (Posidonia oceanicatandem repeat). Finally, the ability of PoTR to detect genetic variability inP. oceanicagenome was demonstrated by the presence of amplification products of different lengths utilizing primers internal to this sequence. |
| Databáze: |
Supplemental Index |
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