| Autor: |
Laffargue, Philippe, Fialdes, Patrice, Frayssinet, Patrick, Rtaimate, Mohamed, Hildebrand, Hartmut F., Marchandise, Xavier |
| Zdroj: |
Journal of Biomedical Materials Research; March 2000, Vol. 49 Issue: 3 p415-421, 7p |
| Abstrakt: |
In order to develop bone substitutes, the design of biomaterials like calcium phosphate ceramic loaded with bone growth factor are of great interest. However, it is necessary to control the amount of growth factor adsorbed onto ceramics and the kinetics of its release. Radiolabeling of insulinlike growth factorI IGFI with 125iodine 125IIGFI and its adsorption onto porous tricalcium phosphate TCP cylinders enabled us to establish the timeadsorption and timerelease curves using various concentrations of IGFI. The adsorption curve increased rapidly and then flattened out at 72 h; 90 of the maximum was already reached at 24 h; and 20 of the adsorbed IGFI was released in water within 4 days. In human serum the release was faster at 82 within 4 days. In vivoevaluation on an animal model was then performed. Rabbits bilateral femoral cylindrical bone defects were filled with the TCP cylinders, which were either carrying IGFI or implanted alone as a control in each rabbit. Bone turnover and ceramic resorption were stimulated by IGFI loaded TCP according to standard radiography, dualenergy Xray absorptiometry, histology, and histomorphometry. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 49, 415–421, 2000. |
| Databáze: |
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