| Autor: |
Bos, Gert W., Scharenborg, Nicole M., Poot, André A., Engbers, Gerard H. M., Beugeling, Tom, van Aken, Willem G., Feijen, Jan |
| Zdroj: |
Journal of Biomedical Materials Research; March 1999, Vol. 44 Issue: 3 p330-340, 11p |
| Abstrakt: |
Seeding of endothelial cells ECs on the luminal surface of smalldiameter vascular grafts is a promising method to avoid occlusion of these prostheses. Immobilization of basic fibroblast growth factor bFGF to substrates used to coat or fill porous prostheses may enhance the formation of a confluent monolayer of ECs. Human umbilical vein endothelial cells HUVECs were grown on bFGFloaded albumin–heparin conjugate bound to CO2gasplasmatreated polystyrene. In the order of 2–3 ngcm2bFGF had to be immobilized to form a confluent monolayer of HUVECs. The most prominent effect of surfaceimmobilized bFGF was stimulation of the proliferation shortly after seeding, resulting within 3 days in confluent cell monolayers with high density. In contrast, in cultures with 0.3 ngmL bFGF in the medium instead of bFGF bound to the surface, it took almost a week before the cell layers reached confluency. Binding of bFGF to heparin and the biological activity of bFGF towards ECs were not influenced by the radiolabeling of bFGF with iodine. However, only a minor part of the bFGF used in this study displayed heparin affinity. Furthermore, degradation and multimerization of labeled bFGF in time occurred when the growth factor was stored at 20°–37°C. This limits the use of labeled bFGF to shortterm hours experiments. In conclusion, bFGF loading of vascular graft surfaces through complexation of bFGF with a heparincontaining matrix probably will lead to more rapid formation of a confluent monolayer of ECs on graft surfaces upon seeding of the cells. © 1999 John Wiley & Sons, Inc. J Biomed Mater Res, 44, 330–340, 1999. |
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