| Abstrakt: |
Environmental isolations of the dimorphic fungus, Blastomyces dermatitidis, the aetiologic agent of blastomycosis are rare, and have usually involved animal innoculation techniques. We report the in vitroisolation of B. dermatitidisfrom a woodpile in November 1997, from a private property in a highly endemic area of north central Wisconsin, USA. The woodpile was 73m from the Wisconsin River and 5m from a kennel which had housed nine dogs over the past 14 years, four of which had been diagnozed with blastomycosis. One of 19 samples from the property yielded B. dermatitidisafter 37°C incubation in a neutral aqueous solution of allantoin, Tween-80, potassium phosphate, magnesium sulphate, penicillin and streptomycin followed by plating on yeast-extract phosphate agar at 20°C. Refinements of this technique may help further elucidate the ecological niche of B.dermatitidis. |
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