| Autor: |
Lawrie, Denise, Coetzee, Lindi M., Glencross, Deborah K. |
| Zdroj: |
Cytometry Part B: Clinical Cytometry; May 2010, Vol. 78 Issue: 3 p201-210, 10p |
| Abstrakt: |
Background:Bead count rate BCR monitoring successfully identifies pipetting error during single platform CD4 enumeration. Despite rigorous prescribed quality control performed, preliminary data suggested that BCR outliers could also be attributed to occasional failure of flow cytometric volumetric operation. The aim of this report was to use counting beads in a model of continuous quality control CQC to monitor overall flow cytometric performance laser alignment, fluorescence stability and volumetric operation.Methods:The proposed CQC model used FlowCheck™ and IMMUNOTROL™ blood controls daily. Extended monitoring of fluidics FPV; beads and sheath only and sample preparation SPV; blood, IMMUNOPREP™ and beads was done daily on five flow cytometers over five consecutive days prior to testing patient samples. Sampletosample CQC included monitoring BCR, selected timefluorescence histograms Time vs. Count; Time vs. Fluorescence and Forward Scatter vs. Fluorescence and full peak coefficient of variation FPCV for 2000 samples tested.Results:Prescribed quality controls showed Half Peak CV values of <2 FlowCheck with Immunotrol within 0.5SD of the target means. Laser stability was confirmed FPCV values <2. However, fluidics volumetric operation fluctuated as indicated by a 3.2 BCR outlier rate of 2,000 samples tested minus pipetting error despite optimal fluidics performance verified at startup FPV CV < 3.Conclusions:Sustained laser stability was confirmed with Time vs. Fluorescence histograms, but Time vs. Count histograms were insufficient to detect intermittent volumetric failure. The proposed CQC model, incorporating BCR monitoring with timefluorescence histograms and FPCV monitoring can identify all volumetric inconsistencies in realtime. © 2010 Clinical Cytometry Society |
| Databáze: |
Supplemental Index |
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