| Autor: |
Sha Valli Khan, P.S., Kozai, T., Nguyen, Q.T., Kubota, C., Dhawan, V. |
| Zdroj: |
Biologia Plantarum; March 2003, Vol. 46 Issue: 2 p161-166, 6p |
| Abstrakt: |
The growth and water relations of Paulownia fortuneiin photoautotrophic cultures (nutrient medium lacking sucrose and growth regulator) with CO2enrichment (PWAH) or without CO2enrichment (PWAL) were compared with those in photomixotrophic shoot (PWC; 30 g dm−3sucrose and 0.3 mg dm−3N6-benzyladenine) and root cultures (PWR; 0.3 mg dm−3indole-3-butyric acid). The photoautotrophic and photomixotrophic cultures were incubated under photosynthetic photon flux 125 and 60 μmol m−2s−1, respectively. 100 % sprouting and significantly higher number of shoots (1.6) were obtained with PWAH as compared to PWAL and PWC. PWAH and PWAL stimulated spontaneous rooting from the cut end of axillary shoots. In PWAH, 84 % of shoots rooted with an average of 5.9 roots per shoot and 4.0 cm of root length in 21 d. Rooting of photomixotrophic shoot cultures were stimulated by an auxin treatment. In this case, 98.3 % of shoots were rooted with an average of 4.6 roots per shoot and 1.9 cm length. A microscopic observation on leaf abaxial surface prints from photomixotrophic shoot and root cultures showed widely open (6 – 8 μm) spherical stomata (12 – 14 μm) and from photoautotrophic cultures elliptical stomata (10 – 12 μm) with narrow openings (3 – 4 μm). Leaves from photomixo-trophic cultures had higher stomatal index as compared to photoautotrophic cultures. The rate of moisture loss from detached leaves was not varying significantly in different cultures. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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