| Autor: |
Roode, Mattheus De, Peters, Sjors W. P. G., Franssen, Maurice C. R., Padt, Albert Der Van, Groot, Aede De, Boom, Remko M. |
| Zdroj: |
Biotechnology & Bioengineering; 5 March 2001, Vol. 72 Issue: 5 p568-572, 5p |
| Abstrakt: |
This article describes the synthesis of glyceryl glucoside from glycerol and glucose with almond β-glucosidase as the catalyst. A yield of 54% (0.45 mmol/g) was obtained. The influence of the enzyme stability, the water concentration, and the water activity on the glucoside yield were determined. A molar fraction-based equilibrium constant of 2.4 ± 0.6 was found, with which the glucoside yield could be calculated for all possible combinations of initial substrate and water fractions in the reaction mixture. A model was used to optimize the glucoside yield while minimizing one of the substrate concentrations at equilibrium. This straightforward model gives a good prediction of the measured glucoside yield, according to a parity plot. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 568572, 2001. |
| Databáze: |
Supplemental Index |
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