| Autor: |
Ioos, Renaud, Laugustin, Lise, Schenck, Nathalie, Rose, Sylvie, Husson, Claude, Frey, Pascal |
| Zdroj: |
European Journal of Plant Pathology; October 2006, Vol. 116 Issue: 2 p171-176, 6p |
| Abstrakt: |
Introns are generally highly polymorphic regions within genes and were proven to be of great interest for discriminating among phylogenetically-close Phytophthoraspecies. Phytophthora ramorumand P. fragariaeare considered as quarantine pathogens by the European Union and accurate detection tools are therefore necessary for their monitoring. From introns located in different single copy genes (GPA1, RAS-like, and TRP1), we developed a series of PCR primers specific to P. ramorumand P. fragariae. The specificity of these primers was successfully checked with a wide collection of Phytophthoraisolates and a protocol was developed to detect both pathogens directly in infected plant tissues. These genes should be of particular interest for the development of additional species-specific detection tools within the Phytophthoragenus.Introns are generally highly polymorphic regions within genes and were proven to be of great interest for discriminating among phylogenetically-close Phytophthoraspecies. Phytophthora ramorumand P. fragariaeare considered as quarantine pathogens by the European Union and accurate detection tools are therefore necessary for their monitoring. From introns located in different single copy genes (GPA1, RAS-like, and TRP1), we developed a series of PCR primers specific to P. ramorumand P. fragariae. The specificity of these primers was successfully checked with a wide collection of Phytophthoraisolates and a protocol was developed to detect both pathogens directly in infected plant tissues. These genes should be of particular interest for the development of additional species-specific detection tools within the Phytophthoragenus. |
| Databáze: |
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