| Autor: |
Bucek, Pavel, Jaumot, Joaquim, Aviñó, Anna, Eritja, Ramon, Gargallo, Raimundo |
| Zdroj: |
Chemistry - A European Journal; November 2009, Vol. 15 Issue: 46 p12663-12671, 9p |
| Abstrakt: |
Guanine‐rich regions of DNA are sequences capable of forming G‐quadruplex structures. The formation of a G‐quadruplex structure in a region 140 base pairs (bp) upstream of the c‐kittranscription initiation site was recently proposed (Fernando et al., Biochemistry, 2006, 45, 7854). In the present study, the acid–base equilibria and the thermally induced unfolding of the structures formed by a guanine‐rich region and by its complementary cytosine‐rich strand in c‐kitwere studied by means of circular dichroism and molecular absorption spectroscopies. In addition, competition between the Watson–Crick duplex and the isolated structures was studied as a function of pH value and temperature. Multivariate data analysis methods based on both hard and soft modeling were used to allow accurate quantification of the various acid–base species present in the mixtures. Results showed that the G‐quadruplex and i‐motif coexist with the Watson–Crick duplex over the pH range from 3.0 to 6.5, approximately, under the experimental conditions tested in this study. At pH 7.0, the duplex is practically the only species present. |
| Databáze: |
Supplemental Index |
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