Direct non-invasive observation of metabolism in living cells by 13C nuclear magnetic resonance spectroscopy

Autor: Burton, Gerardo, Baxter, Robert L., Gunn, J. Martyn, Sidebottom, Philip J., Fagerness, Paul E., Shishido, Kozo, Lee, John Y., Scott, A. Ian
Zdroj: Canadian Journal of Chemistry; September 1980, Vol. 58 Issue: 17 p1839-1846, 8p
Abstrakt: Applications of 13C nmr in following the metabolism and fate of 13C enriched substrates in whole cells are described. Studies on the elaboration of coproporphyrinogens I (2a) and III (2b) from [5-13C]-δ-aminolevulinic acid (1) and [11-13C-porphobilinogen (PBG, 3), in Rhodopseudomonasspheroidesand Propionibacteriumshermanii, respectively, the randomization of 13C from [3-13C]-propionate by P. shermanii, the biosynthesis of citrate, gentisalcohol (4), and patulin (5) from [2-13C]-acetate by Penicilliumurticae, and the anaerobic metabolism of [1-13C]-glucose in rabbit erythrocytes are reported.
Databáze: Supplemental Index