| Autor: |
BIEKER, J. J., OUYANG, L., CHEN, X. |
| Zdroj: |
Annals of the New York Academy of Sciences; June 1998, Vol. 850 Issue: 1 p64-69, 6p |
| Abstrakt: |
Correct temporal control of the ?-like globin cluster is generated in part by the binding of tissue-restricted transcriptional regulators to their cognate sites. Erythroid Krüppel-like Factor (EKLF) is one of these red cell-specific activators that is particularly important for switching on adult ?-globin gene expression. However, its simple presence is not sufficient to activate the ?-globin promoter, as primitive erythroid cells and a number of erythroid cell lines express EKLF yet do not express adult ?-globin. One explanation that may account for these observations is that post-translational modification of EKLF differs within these cell populations. To address this issue, we are investigating whether phosphorylation plays a role in modulating EKLF activity. In vitroand in vivoapproaches have been used to demonstrate that EKLF is a phosphoprotein whose ability to bind DNA and transcriptionally activate an adjacent promoter is critically dependent on its phosphorylation status. Of particular interest is a casein kinase II site within the EKLF minimal transactivation domain. |
| Databáze: |
Supplemental Index |
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