| Autor: |
Laga, Richard, Koák, estmír, Šubr, Vladimír, Ulbrich, Karel |
| Zdroj: |
Macromolecular Chemistry and Physics; July 2009, Vol. 210 Issue: 14 p1138-1148, 11p |
| Abstrakt: |
New, hydrophilic polymers and copolymers based on HPMA, carrying reactive thiazolidine2thione groups either situated at the polymer chain end or randomly distributed along the chains bound to the polymer backbone via spacers containing reductively degradable disulfide linkages, have been developed for an efficient and intracellularly removable coating of genedelivery vectors. Aminogroupbearing PSlatex nanoparticles and selfassembled polyLlysineDNA complexes were used as models for genedelivery viral and nonviral vectors to study the coating reaction with reactive HPMAbased copolymers. It is shown that modification of the particles was effective, resulting in an increase in the $\overline M _{\rm w} $and Rhof the particles, which depend on the polymer length and its detailed structure. Incubation of the coated particles in buffers modeling the intracellular environment induced unshielding of the vectors and release of the polymer layer from their surface. |
| Databáze: |
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