| Autor: |
Ivessa, Andreas S, Zhou, Jin-Qiu, Schulz, Vince P, Monson, Ellen K, Zakian, Virginia A |
| Zdroj: |
Genes & Development; June 2002, Vol. 16 Issue: 11 p1383-1396, 14p |
| Abstrakt: |
In wild-type Saccharomyces cerevisiae, replication forks slowed during their passage through telomeric C(1-3)A/TG(1-3) tracts. This slowing was greatly exacerbated in the absence of RRM3, shown here to encode a 5' to 3' DNA helicase. Rrm3p-dependent fork progression was seen at a modified Chromosome VII-L telomere, at the natural X-bearing Chromosome III-L telomere, and at Y'-bearing telomeres. Loss of Rrm3p also resulted in replication fork pausing at specific sites in subtelomeric DNA, such as at inactive replication origins, and at internal tracts of C(1-3)A/TG(1-3) DNA. The ATPase/helicase activity of Rrm3p was required for its role in telomeric and subtelomeric DNA replication. Because Rrm3p was telomere-associated in vivo, it likely has a direct role in telomere replication. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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