| Autor: |
Ketting, R F, Fischer, S E, Bernstein, E, Sijen, T, Hannon, G J, Plasterk, R H |
| Zdroj: |
Genes & Development; October 2001, Vol. 15 Issue: 20 p2654-2659, 6p |
| Abstrakt: |
Double-stranded RNAs can suppress expression of homologous genes through an evolutionarily conserved process named RNA interference (RNAi) or post-transcriptional gene silencing (PTGS). One mechanism underlying silencing is degradation of target mRNAs by an RNP complex, which contains approximately 22 nt of siRNAs as guides to substrate selection. A bidentate nuclease called Dicer has been implicated as the protein responsible for siRNA production. Here we characterize the Caenorhabditis elegans ortholog of Dicer (K12H4.8; dcr-1) in vivo and in vitro. dcr-1 mutants show a defect in RNAi. Furthermore, a combination of phenotypic abnormalities and RNA analysis suggests a role for dcr-1 in a regulatory pathway comprised of small temporal RNA (let-7) and its target (e.g., lin-41). |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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