| Autor: |
Behr, Matthias, Schieferdecker, Kerstin, Bühr, Patrick, Büter, Marco, Petsophonsakul, Wilaiwan, Sirirungsi, Wasna, Redmann-Müller, Ingrid, Müller, Ulrike, Prempracha, Nalinee, Jungwirth, Christoph |
| Zdroj: |
Journal of Interferon & Cytokine Research; November 1, 2001, Vol. 21 Issue: 11 p981-990, 10p |
| Abstrakt: |
To elucidate the host cell defense mechanisms in response to Sindbis viral infection, we have started to characterize interferon (IFN)-stimulated response element (ISRE)-binding proteins activated in infected cells that are involved in the transcriptional induction of IFN type I-inducible genes. Using electromobility shift assays (EMSA), we detected several protein complexes with a human IFN-stimulated gene 15 (ISG15) ISRE in extracts from virus-infected L929 cells that were absent in extracts from uninfected cells. Comigration with Newcastle disease virus-activated ISRE-binding complexes, ISRE-binding specificity, supershift experiments, and conditions of formation indicate that the complexes activated by Sindbis viral infection in L929 cells correspond to DRAF1 and ISG factor 3 (ISGF3). Transfection of L929 cells with poly rI:rC induced only ISGF3. DRAF1 could be detected in Sindbis virus-infected mouse embryo fibroblasts derived from IFNR type I and type II KO mice. Viral RNA synthesis is required for activation of DRAF1. |
| Databáze: |
Supplemental Index |
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