| Autor: |
Macreadie, I G, Novitski, C E, Maxwell, R J, John, U, Ooi, B G, McMullen, G L, Lukins, H B, Linnane, A W, Nagley, P |
| Zdroj: |
Nucleic Acids Research; July 1983, Vol. 11 Issue: 13 p4435-4451, 17p |
| Abstrakt: |
A mitochondrial gene (denoted aap1) in Saccharomyces cerevisiae has been characterized by nucleotide sequence analysis of a region of mtDNA between the oxi3 and oli2 genes. The reading frame of the aap1 gene specifies a hydrophobic polypeptide containing 48 amino acids. The functional nature of this reading frame was established by sequence analysis of a series of mit- mutants and revertants. Evidence is presented that the aap1 gene codes for a mitochondrially synthesized polypeptide associated with the mitochondrial ATPase complex. This polypeptide (denoted subunit 8) is a proteolipid whose size has been previously assumed to be 10 kilodaltons based on its mobility on SDS-polyacrylamide gels, but the sequence of the aap1 gene predicts a molecular weight of 5,815 for this protein. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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