| Autor: |
Jensen, E G, Hellung-Larsen, P, Frederiksen, S |
| Zdroj: |
Nucleic Acids Research; January 1979, Vol. 6 Issue: 1 p321-330, 10p |
| Abstrakt: |
In an attempt to establish which RNA polymerase catalyzes the synthesis of the low molecular weight RNA components A, C and D, Ama 1 cells (mutant Chinese hamster cells) were used in experiments with addition of alpha-amanitin. Ama 1 cells contain an altered RNA polymerase II which is 800 times more resistant towards inhibition by alpha-amanitin than the wild type enzyme. Alpha-amanitin (up to 200 microgram/ml) added to these cells does not affect the synthesis of the low molecular weight RNAs A, C and D. These data together with our previous data showing that alpha-amanitin (0.5 - 5.0 microgram/ml) preferentially inhibits the synthesis of A, C and D in normal cells indicate that RNA polymerase II catalyzes the synthesis of the low molecular weight RNA components A, C and D. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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