| Autor: |
Lollini, Pier-Luigi, Palmieri, Gabriella, Giovanni, Carla De, Landuzzi, Lorena, Nicoletti, Giordano, Rossi, Ilaria, Griffoni, Cristiana, Frabetti, Flavia, Scotlandi, Katia, Benini, Stefania, Baldini, Nicola, Santoni, Angela, Nanni, Patrizia |
| Zdroj: |
International Journal of Cancer; 29 May 1997, Vol. 71 Issue: 5 p732-736, 5p |
| Abstrakt: |
Interleukin 15 (IL-15) is a recently discovered cytokine that stimulates lymphocyte proliferation and migration via a trimeric receptor sharing the β and γ signal transducing chains with the IL-2 receptor. IL-15 is typically produced by normal cells that do not release IL-2, but little information is currently available on human tumors. To assess whether human musculo-skeletal sarcomas produce IL-15, we analyzed surgical specimens and cell lines obtained from rhabdomyosarcoma, osteosarcoma and Ewing's sarcoma. IL-15 mRNA was present in 9/9 surgical specimens (3 Ewing's sarcomas, 5 osteosarcomas and 1 rhabdomyosarcoma). The analysis of a panel of cell lines (7 derived from Ewing's sarcoma, 12 from osteosarcoma and 5 from rhabdomyosarcoma) showed that all rhabdomyosarcoma and osteosarcoma cell lines expressed IL-15 mRNA at levels ranging from low to high, while Ewing's sarcoma cells contained little or no IL-15 message. ELISA assays showed IL-15 release in a subset of rhabdomyosarcomas and osteosarcomas, but not in Ewing's sarcoma. The highest production of IL-15, in the picogram/ml range, was found in rhabdomyosarcoma cell lines RH30 and RD. Int. J.Cancer 71:732-736, 1997. © 1997 Wiley-Liss Inc. |
| Databáze: |
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