Fluorescent properties of amino acids labeled with ortho -aminobenzoic acid
Autor: | Ito, Amando S., Turchiello, Rozane De F., Hirata, Isaura Y., Cezari, Maria Helena S., Meldal, Morten, Juliano, Luiz |
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Zdroj: | Biospectroscopy; 1998, Vol. 4 Issue: 6 p395-402, 8p |
Abstrakt: | ortho-Aminobenzoic acid (Abz) has been used as a convenient fluorescent donor group in internally quenched fluorescent peptides, which are employed as substrates for several proteolytic enzymes. As Abz is usually bound to the N-amino terminal of these peptides, it is of interest to investigate the Abz group fluorescent properties bound to different amino acids. We report in this article the optical absorption and fluorescent properties, in aqueous media, of Abz bound to the α-amino group of Ala, Gly, Leu, Ile, Val, Pro, Phe, Arg, Glu, Met, Asn, Tyr, and Trp, with monomethyl-amidated α-carboxyl group. In order to explore the origin of the drastic reduction of Abz attached to Nα amino group of prolyl-peptides, we also examined the fluorescence properties of Abz-NHCH |
Databáze: | Supplemental Index |
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