| Autor: |
Sun, Mao, Louie, Donna, Serwer, Philip |
| Zdroj: |
Biophysical Journal; September 1999, Vol. 77 Issue: 3 p1627-1637, 11p |
| Abstrakt: |
Bacteriophage T7 packages its double-stranded DNA genome in a preformed protein capsid (procapsid). The DNA substrate for packaging is a head-to-tail multimer (concatemer) of the mature 40-kilobase pair genome. Mature genomes are cleaved from the concatemer during packaging. In the present study, fluorescence microscopy is used to observe T7 concatemeric DNA packaging at the level of a single (microscopic) event. Metabolism-dependent cleavage to form several fragments is observed when T7 concatemers are incubated in an extract of T7-infected Escherichia coli (in vitro). The following observations indicate that the fragment-producing metabolic event is DNA packaging: 1) most fragments have the hydrodynamic radius (RH) of bacteriophage particles (±3%) when RH is determined by analysis of Brownian motion; 2) the fragments also have the fluorescence intensity (I) of bacteriophage particles (±6%); 3) as a fragment forms, a progressive decrease occurs in both RH and I. The decrease in I follows a pattern expected for intracapsid steric restriction of 4′,6-diamidino-2-phenylindole (DAPI) binding to packaged DNA. The observed in vitro packaging of a concatemer’s genomes always occurs in a synchronized cluster. Therefore, the following hypothesis is proposed: the observed packaging of concatemer-associated T7 genomes is cooperative. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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