| Autor: |
Fonseca, Luiz Marcos da, Yavo, Boni, Catalani, Luiz Henrique, Falcão, Roberto P., Brunetti, Iguatemy Lourenço, Campa, Ana |
| Zdroj: |
Luminescence: Journal of Biological and Chemical Luminescence; July-August 1998, Vol. 13 Issue: 4 p195-200, 6p |
| Abstrakt: |
Esterase from monocytes promotes the hydrolysis of 2-methyl-1-propenylbenzoate (MPB) yielding 2-methyl-1-propenol, which is oxidized by horseradish peroxidase/H2O2 producing triplet acetone. The chemiluminescence of this reaction can be enhanced by the addition of 9,10-dibromoanthracene-2-sulphonate. The non-specific esterase present in monocytes is responsible for MPB hydrolysis, since (a) the chemiluminescence of the reaction was inhibited by fluoride, and (b) cells that do not contain a significant amount of non-specific esterases, e.g. lymphocytes and neutrophils, did not trigger light emission. The analytical application of this reaction is considered. © 1998 John Wiley & Sons, Ltd. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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