| Autor: |
Shimoyama, Ritsuko, Ohkubo, Tadashi, Sugawara, Kazunobu |
| Zdroj: |
Biomedical Chromatography; August 1999, Vol. 13 Issue: 5 p370-372, 3p |
| Abstrakt: |
An HPLC method was developed for the determination of zonisamide in human breast milk and plasma. Chromatographic separation was achieved using a Develosil CN analytical column with potassium dihydrogenphosphate buffer (pH 3.5 with milk, pH 2.5 with plasma)acetonitrile as the mobile phase. Zonisamide and 1,2-benzisoxazole-3-methansulfonamine acetate as internal standard were detected by ultraviolet absorbance at 240 nm. Zonisamide in breast milk and plasma was extracted by a rapid and simple procedure based on C18 bonded-phase extraction. Determination of zonisamide in human breast milk and plasma was possible in the concentration range 0.0520.0 µg/mL. The recoveries of zonisamide added to human breast milk and plasma were 79.585.0% and 86.393.1%, respectively, with coefficients of variation of less than 8.3% and 11.4% respectively. The mean concentrations of zonisamide in breast milk and plasma were 9.41 ± 0.95 and 10.13 ± 0.45 µg/mL, respectively. The average ratio between the breast milk concentration and plasma concentration (M/P ratio) was 0.93 ± 0.09. Copyright © 1999 John Wiley & Sons, Ltd. |
| Databáze: |
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