| Autor: |
Sizova, Olga I., Kochetkov, Vladimir V., Validov, Shamil Z., Boronin, Alexander M., Kosterin, Paul V., Lyubun, Yelena V. |
| Zdroj: |
Journal of Soils and Sediments; March 2002, Vol. 2 Issue: 1 p19-23, 5p |
| Abstrakt: |
Sorghum was inoculated withPseudomonasbacteria, including strains harboring an As-resistance plasmid, pBS3031, to enhance As-extraction by the plants.Pseudomonasstrains (P.fluorescens38a, P.putida53a, and P.aureofaciensBS1393) were chosen because they are antagonistic to a wide range of phyto-pathogenic fungi and bacteria, and they can stimulate plant growth. The resistance of natural rhizospheric pseudomonads to sodium arsenite was assessed. Genetically modifiedPseudomonasstrains resistant to As(III)/As(V) were obtained via conjugation or transformation. The effects of the strains on the growth of sorghum on sodium-arsenite-containing soils were assessed. The conclusions from this study are: (1) It is possible to increase the survivability of sorghum growing in sodium-arsenite-containing soil by using rhizosphere pseudomonads. (2) The presence of pBS3031 offers the strains a certain selective advantage in arsenite-contaminated soil. (3) The presence of pBS3031 impairs plant growth, due to the As-resistance mechanism determined by this plasmid: the transformation of the less toxic arsenate into the more toxic, plant-root-available arsenite by arsenate reductase and the active removal of arsenite from bacterial cells. (4) Such a mechanism makes it possible to develop a bacteria-assisted phytoremediation technology for the cleanup of As-contaminated soils and is the only possible way of removing the soil-sorbed arsenates from the environment. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full text from SpringerLink