| Abstrakt: |
Summary Distemper virus titrations of 2-, 4-, 5-, and 10-fold steps, each using 5, 8, 10, and 15 C.A.M.'s per dilution, showed differences in the standard error of the median effective dose and mean regression coefficients. For reasonable results, a titration using 2-fold dilutions and eight C.A.M.'s per dilution, repeated three times, was selected as a design for laboratory use. Probit analysis of the titration showed a MED of 4.7±0.076 log10 virus dilution. A 95% confidence interval of the median effective dose was 0.30 log10 virus dilution with 81% of the endpoints occurring 0.10 log10 virus dilution on either side of the median effective dose. The regression coefficient was -1.6±0.29 probits per log10 virus dilution when a 1.5 log10 virus dilution range was used, with the median effective dose occurring midway in the virus dilution range. The regression was valid (t=5.6, t[5%, 3df]=3.2) and the data fitted the line (?2=~0.0, ?2[5%, 3 dff]=7.8). |
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