| Autor: |
van de Merbel, N., Stenberg, M., Öste, R., Marko-Varga, G., Gorton, L., Lingeman, H., Th. Brinkman, U. |
| Zdroj: |
Chromatographia; September 1995, Vol. 41 Issue: 5-6 p6-14, 9p |
| Abstrakt: |
A two-dimensional, column liquid chromatographic system is used for the determination of the D- and L-enantiomers of amino acids in biological samples. Separation of the amino acids is first on ion-exchange column by gradient elution with a sodium citrate-sodium chloride buffer. Enantioseparation is by subsequent injection of 3 µl heart-cuts of the individual amino acids onto a second column with a chiral crown ether stationary phase. Finally, fluorescence detection is after post-column labelling of the amino acids using ano-phthalaldehyde-2-mercaptoethanol reagent solution. The high separation power and selectivity of the system allow processing of complex samples with hardly any additional treatment and the determination of small quantities of D-amino acids in the presence of excess L-form. Applicability of the system is illustrated by the determination of D- and L-aspartate, serine, glutamate and alanine in various complex biological samples, such as protein hydrolysates, urine and biotechnological and food samples. Data are given on detectability, repeatability and linearity. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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