| Autor: |
Huisden, R., Ooms, T., Kraak, J., Poppe, H. |
| Zdroj: |
Chromatographia; March 1991, Vol. 31 Issue: 5-6 p263-271, 9p |
| Abstrakt: |
A stationary phase consisting of maltose on a silica support, designed for the separation of proteins, is described. When maltose is coupled to aminopropylsilica an effective shielding of the silica surface can be obtained. A size exclusion experiment is used to investigate pore structure and the behaviour of proteins. The stability of the surface layer is studied, as influenced by silica pretreatments and cross-linking reactions. Inertness is studied via the denaturation of trypsin in columns of different length at a number of temperatures. An example of a derivatization of the maltose layer is given which demonstrates its use as an interactive separation medium. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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