Retention in coupled capillary column supercritical fluid chromatography with lipids as model compounds

Autor: Karlsson, L., Järemo, M., Emilsson, M., Mathiasson, L., Jösson, J.
Zdroj: Chromatographia; October 1993, Vol. 37 Issue: 7-8 p402-410, 9p
Abstrakt: We have investigated to which extent retention data, acquired on single capillary columns, can be used for predicting retention factors in a coupled column system. For this purpose we utilized a model mixture of 18 lipid components with widely different vapor pressures and polarities. The sample was chromatographed on two columns, SB-biphenyl-30 (70% methyl-30% biphenylpolysiloxane) and SB-cyanopropyl-50 (50% methyl-50% cyanopropylsiloxane). Experimental retention factors, acquired in coupled column systems with two columns connected in different order, were thus compared with values calculated from runs on each single column. The agreement between calculated and experimental values generally was better than 5% without any pressure drop correction.
Databáze: Supplemental Index
Externí odkaz: