| Autor: |
Geske, S. M., French, R., Robertson, N. L., Carroll, T. W. |
| Zdroj: |
Archives of Virology; March 1996, Vol. 141 Issue: 3-4 p541-556, 16p |
| Abstrakt: |
Summary A Montana barley yellow dwarf virus (BYDV) isolate, BYDV-RMV-MT, is serologically identical to the New York RMV type isolate (RMV-NY) but differs in aphid transmission phenotype. A purification procedure for BYDV-RMV-MT was developed and cDNAs encompassing the entire coat protein gene and a portion of the putative polymerase gene of both RMV-MT and RMV-NY were cloned and sequenced. Diameters of RMV-MT virions averaged 24.7 nm. Average virus yield was 4.2 mg/kg plant tissue. There was 81% sequence identity between the clones of MT and NY RMV isolates at the nucleotide level. At the amino acid level the polymerase genes were 91% identical to each other and 74% homologous with that of beet western yellow virus. The coat protein amino acid sequences of the two RMV isolates were only 81% identical and, compared to other sequenced luteoviruses, both were most similar to cucurbit aphid-borne yellows virus. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full text from SpringerLink