| Autor: |
Kouroupis, G. M., Leers, W. -D. |
| Zdroj: |
Archives of Virology; December 1974, Vol. 44 Issue: 4 p370-376, 7p |
| Abstrakt: |
Summary An increase in incubation temperature under humid conditions, and a decrease in an incubation time was used to shorten the radioimmunoassay (RIA) method for hepatitis B antigen (HB-Ag) assay. Incubation of the serum to be tested at 47° C for 120 minutes for primary adsorption was as sensitive as the regular test at room temperature (RT°) for 16 hours. In addition, the washing procedure for the removal of labelled antibody was shortened. In order to break hepatitis B antigenantibody (HB-Ag-Ab) complexes before testing the HB-Ag, sera were heated at 56° C for 30 minutes and then retested by RIA. The heating of the sera resulted in a 2 per cent higher yield of HB-Ag positives. Therefore, we recommend that sera be heated before testing for HB-Ag. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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