| Autor: |
Rhim, Johng S., Simizu, Bunsitu, Wiebenga, Ned H. |
| Zdroj: |
Archives of Virology; June 1967, Vol. 21 Issue: 2 p243-252, 10p |
| Abstrakt: |
Summary Propagation of Junin virus, the etiological agent of Argentinian hemorrhagic fever, has been studied in a variety of cell cultures. One line of African green monkey kidney cells (Vero) at the 107th to 165th passage levels supported the multiplication of the virus and developed a marked cytopathic effect (CPE), while no CPE appeared in another line (BS-C-1) at the 34th passage level. CPE was markedly enhanced by rolling the cultures, and the cytocidal nature of virus replication was markedly more pronounced under these conditions. Junin virus also produced well-defined plaques in monolayer cultures of Vero cells under agar overlay. Virus assays obtained by titration endpoint of CPE or by plaque counts in Vero cells were in almost all instances higher than those obtained by titration in suckling mice. A linear relationship was observed between plaque counts and virus concentration. Maximum adsorption was obtained by 120 minutes incubation at 37°C. When the cultures were infected with <0.001 PFU per cell, the maximum virus titer was obtained on the 5th day after inoculation. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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