| Autor: |
Grant, Kathie A., Kenyon, Sarah, Nwafor, Ijeoma, Plowman, June, Ohai, Charles, Halford-Maw, Robin, Peck, Michael W., McLauchlin, Jim |
| Zdroj: |
Foodborne Pathogens & Disease; October 2008, Vol. 5 Issue: 5 p629-639, 11p |
| Abstrakt: |
Clostridium perfringenscarrying the enterotoxin gene is an important cause of both foodborne and non-foodborne diarrheal disease. Rapid identification of isolates carrying the enterotoxin gene is invaluable for outbreak investigation whilst information on the genomic location of the enterotoxin (cpe) gene can improve our understanding of disease transmission. This paper describes the validation of a real-time polymerase chain reaction (PCR) assay for the identification of C. perfringensand assessment of the potential to cause diarrhea, together with an investigation into the genomic location of the cpegenes in isolates from confirmed incidents of C. perfringensdiarrhea. The real-time assay was shown to be specific for the identification of 253 C. perfringenscultures and gave results concordant with those from motility nitrate and lactose gelatine media, the Nagler reaction, and a conventional block-based PCR assay. The cpegene was detected in 223 of 253 C. perfringenscultures isolated in association with human gastrointestinal disease. A subset of cpe-positive C. perfringensisolates associated with separate incidents of diarrheal disease were investigated further for plasmid or chromosomal location of the cpegene using a multiplex PCR assay. The cpegene was plasmid encoded in two isolates from cases of sporadic diarrhea and six isolates from cases of food poisoning. The cpegene from the remaining 11 isolates from different food poisoning outbreaks was found to be chromosomally encoded. One of the C. perfringensstrains with a plasmid encoded cpegene formed spores of high heat resistance and five formed spores that were sensitive to heating. Eight of the isolates with a chromosomal cpegene formed heat-resistant spores, and two formed spores with an intermediate heat resistance. |
| Databáze: |
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