| Autor: |
Wu, Zhuping, Zhang, Chao, Yang, Chengdui, Zhang, Xinrong, Wu, Erruo |
| Zdroj: |
Analyst; December 1, 2000, Vol. 125 Issue: 12 p2201-2205, 5p |
| Abstrakt: |
A selective, reliable and rapid method for the simultaneous determination of progesterone and norgestrel concentrations in human serum after taking oral contraceptive tablet has been developed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) with atmospheric pressure chemical ionization (APCI) interface. The norethisterone was used as the internal standard. Selected transitions of m/z 313/245 for norgestrel, m/z 315/97 for progesterone and m/z 299/109 for norethisterone were monitored using multiple reaction monitoring (MRM) mode for quantitation. The assay was linear over the concentration range of 0.250 ng mL−1 for norgestrel and progesterone. The lower level of quantitation in human serum was obtained at 0.2 ng mL−1 for both norgestrel and progesterone using optimum tuning parameters. The intra-assay precision and inter-assay precision do not exceed 10 and 9%, respectively. The method has been applied to the determination of norgestrel and progesterone in serum of female volunteers. |
| Databáze: |
Supplemental Index |
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