| Autor: |
Hauck, Michael, Köbler, Helmut |
| Zdroj: |
Zeitschrift für Lebensmitteluntersuchung und -Forschung A; October 1990, Vol. 191 Issue: 4-5 p322-324, 3p |
| Abstrakt: |
Summary A method for the analysis of cyclamate in complex foodstuffs has been developed. This method is applicable in strongly coloured and protein-rich foodstuffs. The quantitative determination depends on oxidation of cyclamate to cyclohexylamine and derivatisation with 4-fluoro-7-nitrobenzofuran (NBD-F). The derivatives are analysed by HPLC on a C18: reversed-phase column, their minimal stability being 12 h. There are two possible methods of detection: (a) absorbance at 485 nm and (b) fluorescence with excication at 485 nm and emission at 530 nm. The detection limit of cyclamate is 5 mg/kg foodstuff, with fluorescence detection 0.4 mg/kg. The recoveries are in the range of 88% to 104%. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
