| Autor: |
Moustafa, Esam K., Stegemann, Hermann, Antonacopoulos, Nicolaus |
| Zdroj: |
Zeitschrift für Lebensmitteluntersuchung und -Forschung A; May 1982, Vol. 175 Issue: 3 p199-202, 4p |
| Abstrakt: |
Summary All proteins from fish including boiled samples can be dissolved at room temperature in Tris-borate-buffer pH 8.9 containing 2% Sodiumdodecylsulfate (SDS) and 1% mercapto-ethanol. The separation is carried out in polyacrylamidegels (PAG) by SDS-electrophoresis, showing a species-specific pattern even for the heated samples. Focussing in PAG after removing SDS by precipitation and separation in the presence of urea and tetramethyl-urea between pH 2 and 11 showed species-specificity and a shift of protein bands which partially mirrored the increasing autolysis and degradation of the samples during storage. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
