Spectrophotometric determination of rosmarinic acid in plant cell cultures by complexation with Fe2+ ions

Autor: López-Arnaldos, T., López-Serrano, M., Barceló, A. Ros, Calderón, A. A., Zapata, J. M.
Zdroj: Fresenius' Journal of Analytical Chemistry; January 1995, Vol. 351 Issue: 2-3 p311-314, 4p
Abstrakt: A spectrophotometric method for determining rosmarinic acid (a-O-caffeoyl-3,4-dihydroxyphenyllactic acid) in unpurified methanolic extracts from Lavandula cell cultures is reported. It is based on a complexation reaction of rosmarinic acid with Fe2+ to give a blue-dark product with ?max=572 nm and ?573=3.82×103 l mol-1 cm-1. The stoichiometry of the reaction and the optimal conditions for colour development are checked. The sensitivity and accuracy of this spectrophotometric method are compared with UV-spectral and HPLC methods for determining rosmarinic acid in plant cell cultures; similar results are obtained.
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