| Autor: |
Vogt, W., Koczorek, Kh. R., Vogt, H. -H., Knedel, M., Rindfleisch, Gerda |
| Zdroj: |
Fresenius' Journal of Analytical Chemistry; January 1974, Vol. 269 Issue: 3 p187-192, 6p |
| Abstrakt: |
Through the azocoupling of Val5-angiotensin II-Asp1-ß-amide (Hypertensin-CIBA) with p-halogene substituted benzenediazonium salts the possibility is shown for quantitative determination of polypeptides containing tyrosine and/or histidine. 1 to 2 nanomoles of the coupling-product could be measured photometrically. The detection limit can be lowered, if the polypeptide is coupled with a radioactive labelled diazonium salt. The preferable reaction conditions for diazotising and coupling are reported. The reaction-product showed an uniform behaviour in the used paper-chromatographic system. Probably it is a monoazo compound. Under the chosen conditions the tyrosyl group is very likely the reacting part of the hypertensin molecule. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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