| Autor: |
Meyer, T., von Wichert, P., Weins, D. |
| Zdroj: |
Journal of Molecular Medicine; February 1989, Vol. 67 Issue: 3 p113-118, 6p |
| Abstrakt: |
Summary Two methods of phospholipase A2 determination using14C-labelledE. coli bacterias as substrate were compared. One method works with a filter membrane for separation of cleaved14C-oleate from remaining phospholipids, the other uses the well-known thin-layer chromatography for lipid analysis. Some features of human serum phospholipase A2 regarding pH and Ca2+ dependency were investigated. Possible sources of errors were discussed. It was shown that either method can differentiate between normal and pathologically elevated phospholipase A2 levels, but that the filter method is superior in terms of sensitivity and workload. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full text from SpringerLink