| Autor: |
Trillo, J. Miñones, Jado, E. Iribarnegaray, Fernández, S. García, Pedrero, P. Sanz |
| Zdroj: |
Colloid & Polymer Science; April 1972, Vol. 250 Issue: 4 p325-329, 5p |
| Abstrakt: |
Summary The influence of pH and ionic strength of the substrate on the characteristics of human serum albumin monolayers are studied to correlate the type of monolayer of protein with its structure. When the pH of the substrate was kept constant (pH 2) thep-A curves were more expanded at low salt concentrations. Nevertheless, if the pH of the substrate corresponds to the I. P. of the protein (I. P.=5.1), an increase in the ionic strength of the same increases the limiting area of the film, without hoticeably changing its physical state. On very acid or alkaline substrates films of H. S.A. were of expanded typs, unlike those spread on neutral, slightly acid or alkaline substrates. The isotherm obtained on sodium salicylate substrate was very similar to the pH 12.5 curve, confirming that under these ph conditions hydrogen bonds of molecule protein are broken. |
| Databáze: |
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| Externí odkaz: |
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